| Isolates and drug susceptibilityPseudomonas aeruginosa isolates have been identified by several tests that included: Gram-negative bacilli, motile, oxidase and catalase good, bluish green pigmentation, and glucose oxidizer. In this study, 40 P. aeruginosa isolates were collected from burn wound samples of 23 male and 17 female hospitalized individuals. The mean ageofthepatientswere26?yearsand21patientshadNeckand face skin wound, 12 sufferers had hands and arms wound and 7 patients had complete body wounds. The highest resistance was detected for cefotaxime, ceftazidime, meropenem, imipenem and piperacillin, and 16 (40 ) isolates had been resistant to these antibiotics. The resistance rate to ciprofloxacin and gentamicin was slightly decrease and 12 (30 ) isolates were resistant to them (Table two). Minimum inhibitory concentrations (MIC) of colistin against the isolates were lower than 2 g/mL,andnoresistancewasseen.AccordingtotheDSTresults, isolates have been categorized to 17 MDR, 13 mono-drug resistance, and ten susceptible isolates.three.5 | Virulence factorsAmongthevirulencegenes,lasB and exoA genes were detected in 38 (95 )isolates.TheothergenesincludingplcH, exoS, and nan1 were present in 37 (92 ), 36 (90 ), and 16 (40 ) isolates, respectively. Althoughwedidnotfindanycorrelationbetweenthevirulenceand -lactamase genes, the co-existence of virulence genes (lasB, exoA, plcH, exoS, and nan1) was observed among the isolates. The algD gene was present in 17 (43 ) isolates and all of them had been sturdy biofilm producers.GHASEMIAN et al.5 of|TA B L E three DistributionofbiofilmformationamongP. aeruginosa isolates and correlation in between biofilm production and antibiotic resistance patterns or co-presence of virulence factors.Antibiotic resistance phenotype Biofilm production Sturdy N ( ) Moderate N ( ) Weak N ( ) Total N ( ) Isolates N ( ) 17 (42.five ) 14 (35 ) 9(22.5 ) 40 (100 ) MDR 11 5 1 17 (42.5 ) 0.045 Mono-drug resistance four six three 13 (32.5 ) S 2 three 5 10 (25 ) Quantity of virulence things 1 0 1 2 3 0.445 2 two 3 1 6 three 5 1 2 8 4 three 1 0 4 five 3 five three 11 six 4 three 1Pearson Chi-square p-valueNote: p-value 0.05 thought of as a important correlation. Abbreviations:MDR,Multi- rugresistant;S,Susceptiblephenotype. dTA B L E four DistributionofESBL,MBL, and carbapenemase genes amongst 40 P. aeruginosa isolates.ESBL Genotypic 16 (40 ) MBL Genotypic 12 (30 ) Carbapenemase Genotypic 16 (40 ) Carba NP 16 (40 ) Phenotypic 12 (30 ) Phenotypic 0 (0 )bla TEM eight (20 ) bla VIM 0 blaOXA-48blaSHV 6 (15 ) blaIMP 0 blaOXA-23 1 (2/5 )blaCTX-M 15 (37/5 ) blaNDM 12 (30 ) blaOXA-11 7 (17/5 )TA B L E 5 Thedetailsofco- resenceofbla genes among P.2-Hydroxyethyl methacrylate Order aeruginosa isolates.DBCO-C6-acid uses p-lactamase class ESBL+MBL ESBL+MBL ESBL+MBL ESBL+MBL ESBL+Carbapenemase ESBL+Carbapenemase ESBL+Carbapenemase MBL+Carbapenemase ESBL+MBL+Carbapenemase ESBL+MBL+Carbapenemase ESBL+MBL+Carbapenemase Total Abbreviation:MDR,Multi- rugResistant.PMID:27217159 D Detection of genes by PCR assays (bla TEM , blaSHV, blaCTX-M)+ (bla VIM) (bla TEM)+(bla VIM) (bla TEM , blaSHV )+(bla VIM) (bla TEM , blaCTX-M)+(bla VIM) (bla TEM)+(blaoxa-48) (bla TEM , blaSHV, blaCTX-M)+(blaoxa-23) (bla TEM , blaSHV )+(blaoxa-48) (bla VIM)+(blaoxa-48) (bla TEM)+(bla VIM)+(blaoxa-48) (bla TEM , blaCTX-M)+(bla VIM)+(blaoxa-48) (bla TEM , blaSHV, blaCTX-M)+(bla VIM)+(blaoxa-48) Isolates (N) two 3 1 1 1 1 1 1 two 1 1 15 Phenotype MDR MDR MDR MDR MDR MDR MDR MDR MDR MDR MDR3.six | ERIC-PCR typingERIC-PCR typing indicated high genetic diversity among the isolates. The results of genotyping showed that 36 is.