N was provided by the National All-natural Science Foundation of China (Nos. 31271812 and 31670064), National Higher Technologies Analysis and Improvement System of China (863 Program No. 2012AA022105C).Publisher’s NoteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Received: two March 2017 Accepted: six AprilReferences Arumugam M, Agarwal A, Arya MC, Ahmed Z (2013) Influence of nitrogen sources on biomass productivity of microalgae Scenedesmus bijugatus. Bioresour Technol 131:24649 Benider A, Tahiri M, Belkoura M, Dauta A (2001) Interacting effect of heliothermic components on the growth price of 3 Scenedesmus species. Int J Limnol 37:25766 Bligh EG, Dyer WJ (1959) A fast method of total lipid extraction and purification. Can J Biochem Physiol 37:91117 Chaney AL, Marbach EP (1962) Modified reagents for determination of urea and ammonia. Clin Chem 8:13032 Chang G, Luo Z, Gu S, Wu Q, Chang M, Wang X (2013) Fatty acid shifts and metabolic activity adjustments of Schizochytrium sp. S31 cultured on glycerol. Bioresour Technol 142:25560 Collos Y, Mornet F, Sciandra A, Waser N, Larson A, Harrison PJ (1999) An opticalmethod for the rapid measurement of micromolar concentrations of nitrate inmarine phy-toplankton cultures. Pure Appl Chem 11:17984 Converti A, Casazza AA, Ortiz EY, Perego P, Del Borghi M (2009) Effect of temperature and nitrogen concentration around the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem Eng Proc 48:1146151 Damiani MC, Popovich CA, Constenla D, Leonardi PI (2010) Lipid analysis in Haematococcus pluvialis to assess its prospective use as a biodiesel feedstock. Bioresour Technol 101:3801807 DeSwaaf ME, Sijtsma L, Pronk JT (2003) High-cell-density fed-batch cultivation on the docosahexaenoic acid generating marine alga Crypthecodinium cohnii. Biotechnol Bioeng 81:66672 Dhup S, Dhawan V (2014) Impact of nitrogen concentration on lipid productivity and fatty acid composition of Monoraphidium sp. Bioresour Technol 152:57275 Fakhry EM, El Maghraby DM (2015) Lipid accumulation in response to nitrogen limitation and variation of temperature in Nannochloropsis salina.4-Chloro-5-cyano-7-azaindole Formula Bot Stud 56:6.Y-27632 (dihydrochloride) Data Sheet doi:ten.1186/s40529-015-0085-7 Gaffney M, O’Rourke R, Murphy R (2014) Manipulation of fatty acid and antioxidant profiles with the microalgae Schizochytrium sp.PMID:23800738 by way of flaxseed oil supplementation. Algal Res 6:195Gong Y, Liu J, Jiang M, Liang Z, Jin H, Hu X, Wan X, Hu C (2015) Improvement of omega-3 docosahexaenoic acid production by marine dinoflagellate Crypthecodinium cohnii utilizing rapeseed meal hydrolysate and waste molasses as feedstock. PLoS 1 ten(5):18 Gonz ez-Garcinu Tabernero A, S chez- varez JM, Martin del Valle EM, Gal MA (2014) Impact of nitrogen source on growth and lipid accumulation in Scenedesmus abundans and Chlorella ellipsoidea. Bioresour Technol 173:33441 Griffiths MJ, Harrison STL (2009) Lipid productivity as a crucial characteristic for selecting algal species for biodiesel production. J Appl Phycol 21:49307 Hamid AA, Mokhtar NF, Taha EM, Omar O, Yusoff WMW (2011) The function of ATP citrate lyase, malic enzyme and fatty acid synthase inside the regulation of lipid accumulation in Cunninghamella sp. 2A1. Ann Microbiol 61:46368 Hao G, Chen H, Wang L, Gu Z, Song Y, Zhang H, Chen W, Chen YQ (2014) Role of malic enzyme for the duration of fatty acid synthesis within the oleaginous fungus Mortierella alpina. Appl Environ Microbiol 80:2672678 Ikaran Z, Su ez-.
